Protein Purification Reagents and Kits
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Filtered Search Results
Thermo Scientific™ HisPur™ Cobalt Purification Kit, 3 mL
Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.
Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 3 mL
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Thermo Scientific™ POROS™ Caprylate Mixed-Mode Cation Exchange Chromatography Resin
Thermo Scientific™ POROS™ Caprylate Mixed-Mode Chromatography Resin is designed to remove moderate to high levels of aggregates during downstream processing of therapeutic antibodies in flow-through mode.
| Particle Size | 50 μm |
|---|---|
| Shipping Condition | Ambient, cold |
| Stationary Phase | Caprylic Acid |
| Type | Resin |
| Target | Monoclonal antibodies |
| Matrix | Cross-linked poly(styrene-divinylbenzene) POROS beads |
| Product Line | POROS |
R&D Systems™ Histidine-tagged Protein Purification Resin
Designed for the immunopurification of His-tagged proteins in a single step
Thermo Scientific™ POROS™ 50 D Weak Anion Exchange Resin
Thermo Scientific™ POROS™ Weak Anion Exchange Media. POROS 50 micron bulk resins is available for low pressure applications, including large scale downstream bioprocessing.
| Shipping Condition | Room Temperature |
|---|---|
| Pore Size | 500 to 10,000 Å |
| Stationary Phase | D |
| Type | Resin |
| Content And Storage | Store at room temperature. |
| Form | Liquid Suspension |
| Binding Strength | Weak |
| Product Line | POROS |
Thermo Scientific™ HisPur™ Ni-NTA Spin Purification Kit, 3 mL
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Cytiva HisTrap™ excel Prepacked Columns
For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.
| Type | Pre-packed Column |
|---|---|
| Content And Storage | 4°C to 30°C |
| Description | HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| pH Range | 2 to 12 |
| Material | Polypropylene |
| Support Type | 6% Highly Cross-linked Agarose |
Cytiva HiScreen™ Q Sepharose™ and SP Sepharose™ Fast Flow IEX Columns
Designed for method optimization and parameter screening
| Buffer | 1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol |
|---|---|
| Height (Metric) | 100mm |
| Content And Storage | 4° to 30°C |
| Diameter (English) Inner | 0.3 in. |
| Bed Volume | 4.7mL |
| Column Type | Ion Exchange Column |
| Diameter (Metric) Inner | 7.7mm |
| Quantity | 1 x 4.7mL |
| Material | Polypropylene |
| For Use With (Application) | Optimization and parameter screening, ion exchange chromato graphy |
| Max. Pressure | 1.5 bar |
| Particle Size | 45 to 165μm |
| Flow Rate | <450 cm/hr. |
| Support Type | 6% Cross-linked Agarose |
| Height (English) | 3.93 in. |
Cytiva Tricorn™ Filter Kit
Used for large volume/repeated loading of clarified sample to reduce the risk of clogging in combination with capture media. Cytiva Tricorn™ Filter Kit includes top and bottom adapters, bottom filters, and EPDM O-rings, five of each
Thermo Scientific™ CaptureSelect™ CH1-XL Affinity Matrix
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Thermo Scientific™ CaptureSelect™ CH1-XL Affinity Matrix is the improved version of CaptureSelect IgG-CH1 Affinity Matrix.
| Ligand Type | CH1-XL affinity ligand |
|---|---|
| Flow Rate | 50 to 200 cm/hr. (Up to 2 bar) |
| Matrix | Agarose-based, epoxide-activated |
| Binding Capacity | >15 mg/mL |
| Form | Agarose Bead |
Cytiva His MultiTrap™ 96-well Filter Plates
Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged proteins
| Type | 96-well Filter Plate |
|---|---|
| Content And Storage | 4°C to 30°C |
| Description | His MultiTrap FF and His MultiTrap HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC) |
| pH Range | 3 to 12 |
| Quantity | 4 x 96-well Filter Plates |
| Material | Polypropylene, Polyethylene |
Thermo Scientific™ POROS™ 50 PI Weak Anion Exchange Resin
Thermo Scientific™ POROS™ Weak Anion Exchange Media. POROS 50 micron bulk resins is available for low pressure applications, including large scale downstream bioprocessing.
| Shipping Condition | Room Temperature |
|---|---|
| Pore Size | 500 to 10,000 Å |
| Stationary Phase | PI (Polyethyleneimine) |
| Type | Bulk Resin |
| Content And Storage | Store at room temperature. |
| Form | Liquid Suspension |
| Binding Strength | Weak |
| Product Line | POROS |
Thermo Scientific™ POROS™ 50 HQ Strong Anion Exchange Resin
Thermo Scientific™ POROS™ Strong Anion Exchange Media. POROS 50 micron bulk resins is available for low pressure applications, including large scale downstream bioprocessing.
| Shipping Condition | Ambient |
|---|---|
| Pore Size | 500 to 10,000 Å |
| Stationary Phase | HQ |
| Content And Storage | Store at 2°C to 30°C. Do not freeze. |
| Product Line | POROS |
Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents
Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.
| Buffer | Lysis Buffer |
|---|---|
| Shipping Condition | Ambient |
| Form | Liquid |
| Product Type | Nuclear and Cytoplasmic Extraction Reagent |
| Reagent Type | Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation |
| Product Line | NE-PER |